Multiplexed Homogeneous Proximity Ligation Assays for High-throughput Protein Biomarker Research in Serological Material
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چکیده
منابع مشابه
Homogeneous and digital proximity ligation assays for the detection of Clostridium difficile toxins A and B
BACKGROUND The proximity ligation assay (PLA) detects proteins via their interaction with pairs of proximity probes, which are antibodies coupled to noncomplementary DNA oligonucleotides. The binding of both proximity probes to their epitopes on the target protein brings the oligonucleotides together, allowing them to be bridged by a third oligonucleotide with complementarity to the other two. ...
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BACKGROUND Sensitive methods are needed for biomarker discovery and validation. We tested one promising technology, multiplex proximity ligation assay (PLA), in a pilot study profiling plasma biomarkers in pancreatic and ovarian cancer. METHODS We used 4 panels of 6- and 7-plex PLAs to detect biomarkers, with each assay consuming 1 microL plasma and using either matched monoclonal antibody pa...
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Löf, L. 2016. Applications of in situ proximity ligation assays for cancer research and diagnostics. Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine 1241. 56 pp. Uppsala: Acta Universitatis Upsaliensis. ISBN 978-91-554-9638-8. In the field of cancer research and diagnostics it is crucial to have reliable methods for detecting molecules involved in the disea...
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ژورنال
عنوان ژورنال: Molecular & Cellular Proteomics
سال: 2011
ISSN: 1535-9476
DOI: 10.1074/mcp.m110.004978